Anti-Undifferentiated hESC Surface Marker monoclonal antibody (DMABT-H21779)

Mouse anti-Human Undifferentiated hESC Surface Marker monoclonal antibody for FC, ICC Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgM
Clone
nbc25
Species Reactivity
Human
Immunogen
Human embryonic stem cells (hESC).
Conjugate
Unconjugated

Applications


Application Notes
Flow Cytometry Analysis: A 1:250 dilution from a previous lot detected an undifferentiated hESC surface marker in human embryonic stem cells.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
hESCs; Mouse Anti-Undifferentiated hESC Surface Marker Monoclonal Antibody; Anti-Undifferentiated hESC Surface Marker Monoclonal Antibody; Undifferentiated hESC Surface Marker Monoclonal Antibody Mouse Anti-Undifferentiated hESC Surface Marker MAb; Anti-U

Product Background


Antigen Description
Human embryonic stem cells (hESCs) are unique cells in that they can be maintained in vitro as pluripotent cells and can be induced to differentiate into many cell types representative to all three germ layers by manipulating culture conditions in vivo. Therefore, hESCs have the potential of providing an unlimited supply of cells for cell-based therapy as well as providing a resource to study basic human developmental biology. Subsequently, much attention has focused on directing hESC differentiation along specific developmental lineages. Cell surface markers specific to undifferentiated hESC cells are necessary in confirming maintenance of pluripotency or to show complete or partial differentiation of the cells in the course of these studies.

Citations


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References


Sidhu, KS; Ryan, JP; et al. Derivation of a new human embryonic stem cell line, Endeavour-2, and its characterization. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 46:269-275(2010).
Ng, VY; Ang, SN; et al. Characterization of Epithelial Cell Adhesion Molecule as a Surface Marker on Undifferentiated Human Embryonic Stem Cells. STEM CELLS 28:29-35(2010).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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