Anti-UBE2L3 monoclonal antibody (DCABH-157)

Rabbit anti-Human UBE2L3 monoclonal antibody for ICC/IF, WB, IP, IHC-P Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
FQS5479
Species Reactivity
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in Human Ube2L3
Conjugate
Unconjugated

Applications


Application Notes
ICC/IF: 1/100 - 1/250; WB: 1/1000 - 1/10000; IP: 1/10 - 1/100; IHC-P: 1/100 - 1/250.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
UBE2L3; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme E2 L3; UBCH7; ubiquitin-protein ligase L3; ubiquitin carrier protein L3
Entrez Gene ID
UniProt ID

Product Background


Gene summary
UBE2L3 (Ubiquitin Conjugating Enzyme E2 L3) is a Protein Coding gene. Diseases associated with UBE2L3 include papilloma and keloid formation. Among its related pathways are Immune System and Remodeling of Adherens Junctions. GO annotations related to this gene include poly(A) RNA binding and ligase activity. An important paralog of this gene is UBE2L6. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Antigen Description
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys-11-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Ubiquitin-conjugating enzyme E2 L3 is a protein that in humans is encoded by the UBE2L3 gene. As an E2 enzyme, UBCH7 participates in ubiquitination to target proteins for degradation. The role of UBCH7 in the ubiquitination of the NF-κB precursor implicated it in various major autoimmune diseases, including rheumatoid arthritis (RA), celiac disease, Crohn’s disease(CD), and systemic lupus erythematosus. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-κB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. UBE2L3 is primarily known for its role in the cell cycle. Specifically, UBE2L3 manages cell cycle regulatory protein levels via the ubiquitin proteolytic pathway (UPP) during the G1/S transition and during the actual S phase. The function about UBE2L3 antigen include ATP binding; acid-amino acid ligase activity; enzyme binding; ligase activity; nucleotide binding; protein binding; transcription coactivator activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity.
Pathway
Adaptive Immune System, organism-specific biosystem; Alpha-synuclein signaling, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem.

Citations


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References


Koo, BK; Chae, S; et al. Identification of Novel Autoantibodies in Type 1 Diabetic Patients Using a High-Density Protein Microarray. DIABETES 63:3022-3032(2014).
Agik, S; Franek, BS; et al. The Autoimmune Disease Risk Allele of UBE2L3 in African American Patients with Systemic Lupus Erythematosus: A Recessive Effect Upon Subphenotypes. JOURNAL OF RHEUMATOLOGY 39:73-78(2012).

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