Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY
MEDIATORS OF INFLAMMATION
Authors: Carvalho-Filho, Paulo C.; Moura-Costa, Lilia F.; Pimentel, Ana C. M.; Lopes, Mabel P. P.; Freitas, Sibelle A.; Miranda, Patricia M.; Costa, Ryan S.; Figueiredo, Camila A. V.; Meyer, Roberto; Gomes-Filho, Isaac S.; Olczak, Teresa; Xavier, Marcia T.; Trindade, Soraya C.
Abstract
This study aimed at evaluating the transcriptional profile of apoptosis-related genes after in vitro stimulation of peripheral blood mononuclear cells (PBMCs) derived from individuals with periodontitis (P) and healthy nonperiodontitis (NP) control subjects with P. gingivalis HmuY protein. PBMCs from the P and NP groups were stimulated with HmuY P. gingivalis protein, and the expression of genes related to apoptosis was assessed by custom real-time polymerase chain reaction array (Custom RT2 PCR Array). Compared with the NP group, the P group showed low relative levels of apoptosis-related gene expression, downregulated for FAS, FAS ligand, TNFSF10 (TRAIL), BAK1, CASP9, and APAF1 after P. gingivalis HmuY protein stimulation. Furthermore, the P group exhibited low levels of relative gene expression, downregulated for CASP7 when the cells were not stimulated. Our data suggest that P. gingivalis HmuY protein might participate differently in the modulation of the intrinsic and extrinsic apoptosis pathways.
Diuron modulates the DNA methylation status of the ILT7 and TRAIL/TNFSF10 genes and decreases the killing activity of plasmacytoid dendritic cells
ENVIRONMENTAL SCIENCES EUROPE
Authors: Briand, Josephine; Joalland, Marie-Pierre; Nadaradjane, Arulraj; Bougras-Cartron, Gwenola; Olivier, Christophe; Vallette, Francois M.; Perruche, Sylvain; Cartron, Pierre-Francois
Abstract
BackgroundPlasmacytoid dendritic cell (pDC) is described as the Swiss knife of immune system. Thus, the understanding of aberrant epigenetic reprogramming of genes governing the pDC functionality by pollutants appears such as an attractive research point.ResultsOur study has investigated the effect of Diuron (an herbicide) on the pDC-killing activity towards cancer cells. Thus, we observed that the Diuron exposure of pDC promotes a context of global DNA hypomethylation, which is associated with a phenotype of decrease of the killing activity of pDC towards cancer cells. At molecular level, our data associated the Diuron-induced global DNA hypomethylation with the elevated expression of TET2, an epigenetic player involved in DNA demethylation processes, and the decrease of the pDC-killing activity with the decrease of TRAIL expression and the increase of ILT7 expression.ConclusionsThus, our article reports that a pollutant (Diuron) induces an epigenetic reprogramming of a subtype of immune cell (pDC), which decreases its killing activity towards tumors cells. In some context, this mechanism might be conducive to the initiation of pathologies.
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