Anti-Thiamphenicol polyclonal antibody

A commercial enzyme-linked immunosorbent assay (ELISA) test has been applied for the qualitative screening analysis of chloramphenicol (CAP) in food of animal origin (muscle, eggs, milk and honey) at level corresponding to the European Union (EU) minimum required performance limit (MRPL) set for CAP analysis. Different analytical protocols were developed for the extraction and purification of CAP from the matrices under investigation. The ELISA kit validation study was performed according to the Commission Decision 2002/657/EC criteria established for qualitative screening methods. In this regard, the following parameters were determined: detection capability (CC beta), specificity/selectivity, applicability/ruggedness/stability. ne CC beta value resulted below the MRPL (0.3 mu g kg(-1)) for all the examined matrices. No relevant interferences from matrix effects or structurally related substances (florfenicol (FF) and thiamphenicol (THP)) were observed. Slight variations of some critical factors in the sample pre-treatment and clean up for muscle were deliberately introduced for ruggedness evaluation and they did not result in any negative effect on the CAP detection. In addition, the recovery rates and precision at three concentration levels (0.30-0.45-0.60 mu g kg(-1)) were evaluated for all matrices under investigation. The proposed method is suitable for qualitative screening analysis of CAP in the above-mentioned food in conformity with the current EU performance requirements. (c) 2004 Elsevier B.V. All rights reserved.

Antibiotic residues in marine sediments of fish farms negatively influence microbial ecologic systems. The microbial degradation of antibiotic residues was experimentally examined in the marine sediments of Uranouchi Bay, to which one of five antibiotics was added. After incubation reducing physical factors, ampicillin, doxycycline, oxytetracycline, and thiamphenicol were significantly degraded, while josamycin maintained most of the initial amounts. The isolates resistant to ampicillin, josamycin, oxytetracycline, or thiamphenicol degraded each antibiotic in wide ranges of degrees, whereas the isolates degrading doxycycline were not obtained. Microbial degradation may contribute to the disappearance of ampicillin, doxycycline, oxytetracycline, and thiamphenicol in the fish farm. In contrast, the disappearance of josamycin would depend on physical factors, but the bacteria degrading josamycin at least exist in the marine sediments. Phylogenetic analysis using 16S rDNA sequences demonstrated that the antibiotic-resistant isolates formed several clusters in the Gram-positive bacterial group, the Flavobacterium-Cytophaga-Bacteroides group, and the proteobacteria subdivisions. The antibiotic-resistant bacterial population would be composed of various species including ubiquitous coastal bacterial groups. Several species of antibiotic resistant bacteria show antibiotic degradation activities, and appear to contribute to the disappearance of antibiotics in Uranouchi Bay.