Human Anti-Tetanus Toxin Heterohybridoma [TB24] (CSC-H1157)

This hybridoma produces mAbs (IgG, kappa light chain) against Tetanus Toxin Datasheet

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General Information


Immunogen
Tetanus toxoid were fused with LTR228 lymphoblastoid cells
Isotype
IgG, kappa light chain
Fusion Species
Human X Human Heterohybridoma
Immunological Donor
Human
Clone
TB24
Cell Line Description
Peripheral blood B cells from a patient that had been immunized with tetanus toxoid were fused with LTR228 lymphoblastoid cells.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
IMDM with 4 mM L-glutamine, 4500 mg/L glucose and 1500 mg/L sodium bicarbonate, supplemented with 10% FBS.
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Target


Introduction
Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

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