Anti-Human Thymidylate Synthase monoclonal antibody (DCABH-165)

Rabbit Anti-Human Thymidylate Synthase monoclonal antibody for IHC-P Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TQ223
Species Reactivity
Human
Immunogen
Synthetic peptide near C-terminus of human TS.
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
IHC-P: 1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Immunohistochemical analysis of Thymidylate Synthase using anti-Thymidylate Synthase Monoclonal Antibody in Colon Adenocarcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

Target


Alternative Names
TYMS; thymidylate synthetase; TS; TMS; HST422; thymidylate synthase
Entrez Gene ID
UniProt ID

Product Background


Gene summary
TYMS (Thymidylate Synthetase) is a Protein Coding gene. Diseases associated with TYMS include timothy syndrome and brugada syndrome 3. Among its related pathways are Metabolism and Cell Cycle, Mitotic. GO annotations related to this gene include protein homodimerization activity and mRNA binding. Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
Antigen Description
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Thymidylate synthetase (EC 2. 1. 45) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). Thymidine is one of the nucleotides in DNA. With inhibition of TS, an imbalance of deoxynucleotides and increased levels of dUTP arise. Both cause DNA damage. The following reaction is catalyzed by thymidylate synthetase:.

Citations


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References


Laurent-Puig, P; Lievre, A; et al. The biological point of view on pharmacogenetics of anticancer agents in colorectal cancer. BULLETIN DU CANCER 95:935-942(2008).
Shebzukhov, YV; Koroleva, EP; et al. Humoral immune response to thymidylate synthase in colon cancer patients after 5-FU chemotherapy. IMMUNOLOGY LETTERS 100:88-93(2005).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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