WB: 1/500 - 1/3000; ICC/IF: 1/100 - 1/200.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
TUBA1A (Tubulin Alpha 1a) is a Protein Coding gene. Diseases associated with TUBA1A include lissencephaly 3 and lissencephaly due to tuba1a mutation. Among its related pathways are Transport to the Golgi and subsequent modification and Cell Cycle, Mitotic. GO annotations related to this gene include GTP binding and structural molecule activity. An important paralog of this gene is TUBA4B. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Lissencephaly 3 (LIS3) [MIM:611603]: A classic type lissencephaly associated with psychomotor retardation and seizures. Features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem. Note=The disease is caused by mutations affecting the gene represented in this entry. Tubulin alpha-1A chain is a protein that in humans is encoded by the TUBA1A gene. 0The function about TUBA1A antigen include GTP binding; GTPase activity; nucleotide binding; protein domain specific binding; structural molecule activity.
Cell Cycle; Cell Cycle, Mitotic; Centrosome maturation; Chaperonin-mediated protein folding; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding; Formation of tubulin folding intermediates by CCT/TriC; G2/M Transition.