General Notes
This product (Catalog No. DPABH-23833) is a rabbit-derived polyclonal antibody raised against human TRAPPC12, targeting the amino acid region 395–735 of the protein. It has been affinity purified by Protein A. TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).
DPABH-23833 is suitable for use in Western blot (WB) (1:500–1:2,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate) applications.
DPABH-23833 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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