Anti-TRA-2-54 monoclonal antibody (DMABT-H21767)

Mouse anti-Human TRA-2-54 monoclonal antibody for FC, IF, IP Datasheet

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Host Species
Antibody Isotype
Species Reactivity
2102Ep human Embryonal Carcinoma Cells


Application Notes
This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP); and hence monitor the differentiation status of EC and ES cells.
FACS Analysis: a starting range of 5-20 μg/mL.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.


Alternative Names
TRA-2-54 antibody; Mouse Anti-TRA-2-54 Monoclonal Antibody; Anti-TRA-2-54 Monoclonal Antibody; TRA-2-54 Monoclonal Antibody Mouse Anti-TRA-2-54 MAb; Anti-TRA-2-54 MAb; TRA-2-54 MAb

Product Background

Antigen Description
The family of alkaline phosphatases in humans is comprised of four members: intestinal, placental, placental-like (germ cell type), and tissue nonspecific. The tissue nonspecific isozyme is also known as the liver/bone/kidney alkaline phosphatase due to its expression in these tissues. It is also highly expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells and is lost as these cells undergo differentiation. The TRA-2-54 (2J) Mouse mAb specifically detects this isozyme and does not detect the other family members, making it a useful tool for tracking the pluripotency status of ES and EC cells in culture.


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Chen, TJ; Sun, LP; et al. Serum OPN Expression for Identification of Gastric Cancer and Atrophic Gastritis and Its Influencing Factors. PLOS ONE 9:-(2014).
Saniee, P; Siavoshi, F; et al. Endocytotic uptake of FITC-labeled anti-H. pylori egg yolk immunoglobulin Y in Candida yeast for detection of intracellular H. pylori. FRONTIERS IN MICROBIOLOGY 6:-(2015).

Custom Antibody Labeling

We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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