Anti-TRA-2-54 monoclonal antibody (DMABT-H21767)
Mouse anti-Human TRA-2-54 monoclonal antibody for FC, IF, IP
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2102Ep human Embryonal Carcinoma Cells
This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP); and hence monitor the differentiation status of EC and ES cells.
FACS Analysis: a starting range of 5-20 μg/mL.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
TRA-2-54 antibody; Mouse Anti-TRA-2-54 Monoclonal Antibody; Anti-TRA-2-54 Monoclonal Antibody; TRA-2-54 Monoclonal Antibody Mouse Anti-TRA-2-54 MAb; Anti-TRA-2-54 MAb; TRA-2-54 MAb
The family of alkaline phosphatases in humans is comprised of four members: intestinal, placental, placental-like (germ cell type), and tissue nonspecific. The tissue nonspecific isozyme is also known as the liver/bone/kidney alkaline phosphatase due to its expression in these tissues. It is also highly expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells and is lost as these cells undergo differentiation. The TRA-2-54 (2J) Mouse mAb specifically detects this isozyme and does not detect the other family members, making it a useful tool for tracking the pluripotency status of ES and EC cells in culture.
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Quintana-Hayashi, MP; Mahu, M; et al. The Levels of Brachyspira hyodysenteriae Binding to Porcine Colonic Mucins Differ between Individuals, and Binding Is Increased to Mucins from Infected Pigs with De Novo MUC5AC Synthesis. INFECTION AND IMMUNITY 83:1610-1619(2015).
Levenstein, S; Rosenstock, S; et al. Psychological Stress Increases Risk for Peptic Ulcer, Regardless of Helicobacter pylori Infection or Use of Nonsteroidal Anti-inflammatory Drugs. CLINICAL GASTROENTEROLOGY AND HEPATOLOGY 13:498-506(2015).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.