Anti-TOP3A polyclonal antibody (DPABH-25482)
Rabbit anti-Human TOP3A (aa 900-1000) polyclonal antibody for WB, ICC/IF
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Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human Topoisomerase III alpha.
WB: 1 μg/ml. ICC/IF: 1 μg/ml.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
TOP3A; topoisomerase (DNA) III alpha; TOP3; ZGRF7; DNA topoisomerase 3-alpha; topo III-alpha
TOP3A (Topoisomerase (DNA) III Alpha) is a Protein Coding gene. Diseases associated with TOP3A include occupational dermatitis. Among its related pathways are Gene Expression and Cell Cycle, Mitotic. GO annotations related to this gene include nucleic acid binding and DNA topoisomerase activity. An important paralog of this gene is TOP3B. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Has DNA decatenation activity. DNA topoisomerase 3-alpha is an enzyme that in humans is encoded by the TOP3A gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. The function about TOP3A antigen include ATP binding; DNA binding; DNA topoisomerase type I activity; protein binding.
Blooms syndrome complex; Cell Cycle; Fanconi anemia pathway; Homologous recombination.
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Mao, Y; Sun, M; et al. SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 97:4046-4051(2000).
Granzen, B; Efferth, T; et al. Differential expression of the drug resistance markers DNA topoisomerase II alpha and glutathione S-transferase-pi in the histological compartments of Wilms' tumors. ANTICANCER RESEARCH 21:771-776(2001).
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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.