Anti-TOP2B monoclonal antibody (DCABH-228)

Rabbit anti-Human TOP2B monoclonal antibody for ICC/IF, WB, IHC-P, ICC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
FQS6488
Species Reactivity
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in Human Topoisomerase II Beta.
Conjugate
Unconjugated

Applications


Application Notes
ICC/IF: 10 μg/ml; WB: 1/10000 - 1/10000; IHC-P: 1/100 - 1/250; ICC: 1/250 - 1/500.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
TOP2B; topoisomerase (DNA) II beta 180kDa; topoisomerase (DNA) II beta (180kD); DNA topoisomerase 2-beta; topo II beta; antigen MLAA-44
Entrez Gene ID
UniProt ID

Product Background


Gene summary
TOP2B (Topoisomerase (DNA) II Beta) is a Protein Coding gene. Among its related pathways are Transport to the Golgi and subsequent modification and SUMOylation. GO annotations related to this gene include protein heterodimerization activity and enzyme binding. An important paralog of this gene is TOP2A. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described.
Antigen Description
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. DNA topoisomerase 2-beta is an enzyme that in humans is encoded by the TOP2B gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents, for example mitoxantrone, and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. The function about TOP2B antigen include ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA-dependent ATPase activity; chromatin binding; enzyme binding; histone deacetylase binding; isomerase activity; nucleotide binding; protein C-terminus binding; protein heterodimerization activi.

Citations


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References


Ovsyannikova, IG; Dhiman, N; et al. Rubella vaccine-induced cellular immunity: evidence of associations with polymorphisms in the Toll-like, vitamin A and D receptors, and innate immune response genes. HUMAN GENETICS 127:207-221(2010).

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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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