Anti-Thyroid Hormone Receptor Alpha 1/2 monoclonal antibody (CABT-51635MH)

Mouse anti-Human Thyroid Hormone Receptor Alpha 1/2 monoclonal antibody for WB

Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
2103
Species Reactivity
Human, Dog, Mouse, Rat
Immunogen
Peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, alpha1/alpha2 isotype.
Conjugate
Unconjugated

Applications


Application Notes
Western Blot: 1/1,000;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
THRA; thyroid hormone receptor, alpha; AR7; EAR7; ERBA; CHNG6
Entrez Gene ID
UniProt ID
P10827

Product Background


Pathway
Endochondral Ossification; Gene Expression; Generic Transcription Pathway; Neuroactive ligand-receptor interaction; Nuclear Receptor transcription pathway; Nuclear Receptors; Thyroid hormone signaling pathway;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


T-3 Induces Both Markers of Maturation and Aging in Pancreatic beta-Cells

DIABETES

Authors: Aguayo-Mazzucato, Cristina; Lee, Terence B., Jr.; Matzko, Michelle; DiIenno, Amanda; Rezanejad, Habib; Ramadoss, Preeti; Scanlan, Thomas; Zavacki, Ann Marie; Larsen, P. Reed; Hollenberg, Anthony; Colton, Clark; Sharma, Arun; Bonner-Weir, Susan

Previously, we showed that thyroid hormone (TH) triiodothyronine (T-3) enhanced -cell functional maturation through induction of Mafa. High levels of T-3 have been linked to decreased life span in mammals and low levels to lengthened life span, suggesting a relationship between TH and aging. Here, we show that T-3 increased p16(Ink4a) (alpha-cell senescence marker and effector) mRNA in rodent and human beta-cells. The kinetics of Mafa and p16(Ink4a) induction suggested both genes as targets of TH via TH receptors (THRs) binding to specific response elements. Using specific agonists CO23 and GC1, we showed that p16(Ink4a) expression was controlled by THRA and Mafa by THRB. Using chromatin immunoprecipitation and a transient transfection yielding biotinylated THRB1 or THRA isoforms to achieve specificity, we determined that THRA isoform bound to p16(Ink4a), whereas THRB1 bound to Mafa but not to p16(Ink4a). On a cellular level, T-3 treatment accelerated cell senescence as shown by increased number of beta-cells with acidic beta-galactosidase activity. Our data show that T-3 can simultaneously induce both maturation (Mafa) and aging (p16(Ink4a)) effectors and that these dichotomous effects are mediated through different THR isoforms. These findings may be important for further improving stem cell differentiation protocols to produce functional beta-cells for replacement therapies in diabetes.

Immune Responses of the Critically Endangered Yangtze Finless Porpoises (Neophocaena asiaeorientalis ssp. asiaeorientalis) to Escalating Anthropogenic Stressors in the Wild and Seminatural Environments

FRONTIERS IN PHYSIOLOGY

Authors: Nabi, Ghulam; Li, Ying; McLaughlin, Richard W.; Mei, Zhigang; Wang, Kexiong; Hao, Yujiang; Zheng, Jinsong; Wang, Ding

Increasing anthropogenic stressors are potential threats to biodiversity conservation and management of Yangtze finless porpoises (YFPs). The objective of this study was to indirectly compare the habitat quality of a natural reserve, Poyang Lake and a seminatural reserve, the Tian-E-Zhou Oxbow (TZO) in terms of anthropogenic stressors by investigating different stress and immunological parameters in the blood of YFPs. Samples from a total of 74 YFPs from the TZO (n = 43) and Poyang Lake (n = 31) were collected and analyzed. The animals were divided into ontogenetic groups: male calf, female calf, juvenile female, juvenile male, and adult male, and reproductive groups: pregnant female, lactating female, and pregnant plus lactating. The blood from all the animals was analyzed for general stress (HSP14, SOD1, TXN, and FTL), metabolic stress (ACAT2 and THRA), and immunity-related genes (IL12p40, IFN gamma, TNF alpha; IL1 alpha, IL1ra, COX2, CRPL, IL4, and IL8) using qPCR. YFPs living in Poyang Lake showed an increased relative expression pattern for IFN gamma, IL1ra, IL4, ACAT2, and CRPL across all the ontogenetic groups with significantly higher expression in adult males. In contrast, YFPs living in the TZO showed a significantly higher expression in 13 of 15 genes analyzed in the male calf group. Across the reproductive states for porpoises living in Poyang Lake, eight of the 15 genes in the pregnant female and three of the 15 genes in the pregnant plus lactating group had a significantly higher expression level. However, in YFPs living in the TZO, eight of the 15 genes showed significantly higher expression in the pregnant and lactating groups. There was significantly a higher expression of most of the genes in porpoises living in the TZO compared to the age-matched groups from porpoises living in Poyang Lake. The exception was the pregnant female group. The higher relative expression of stress and immune genes in the TZO porpoise population compared to porpoises living in Poyang Lake suggests the effects of worsening habitat quality, possibly indicating water pollution and lack of feeding resources.

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