Rabbit Anti-Human THOC2 Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

A synthesized peptide derived from Human THOC2.

Conjugate

Application Notes

IF/ICC 1:100-1:500
IHC 1:50-1:200

General Notes

This product (Catalog No. DPABH-23876) is a rabbit-derived polyclonal antibody raised against human THOC2. This antibody exhibits cross-reactivity with THOC2 from mouse. It has been affinity purified by Protein A. Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. THOC2 (and probably the THO complex) is involved in releasing mRNA from nuclear speckle domains. Required for NXF1 localization to the nuclear rim. Plays a role for proper neuronal development.
DPABH-23876 is suitable for use in IF/ICC 1 (100-1:500), IHC 1 (50-1:200) applications.
DPABH-23876 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Entrez Gene ID

UniProt ID