Anti-THOC1 monoclonal antibody, clone 6F21

Antibody Isotype

IgG2b

Clone

6F21

Species Reactivity

Mouse; Human

Immunogen

Fusion protein containing amino acids 15-374 of human p84 expressed in E. coli.

Conjugate

Application Notes

WB: 0.5 - 2 μg/ml; IP: 0.5 - 2 μg/ml; ICC/IF: 0.5 - 2 μg/ml; Flow Cyt: 1μg/10⁶ cells;

Alternative Names

THOC1; THO complex 1; THO complex subunit 1; HPR1; P84; tho1; hTREX84; nuclear matrix protein p84; P84N5;

Entrez Gene ID

UniProt ID

Antigen Description

Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5 end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposis sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.

Pathway

RNA transport, organism-specific biosystem; RNA transport, conserved biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; THC complex, organism-specific biosystem; TREX complex, organism-specific biosystem;