Anti-TAF5L monoclonal antibody (DCABH-5781)

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
FQS22556
Species Reactivity
Human
Conjugate
Unconjugated

Applications


Application Notes
ICC/IF: 1/100 - 1/250; WB: 1/1000 - 1/10000.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
TAF5L; TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa; TAF5 like RNA polymerase II, p300/CBP associated factor (PCAF) associated factor, 65 kD; TAF5-like RNA polymerase II p300/CBP-associated factor-associated fact
Entrez Gene ID
UniProt ID

Product Background


Gene summary
TAF5L (TATA-Box Binding Protein Associated Factor 5 Like) is a Protein Coding gene. Among its related pathways are Chromatin organization and GPCR Pathway. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and histone acetyltransferase activity. An important paralog of this gene is WDR83. The product of this gene belongs to the WD-repeat TAF5 family of proteins. This gene encodes a protein that is a component of the PCAF histone acetylase complex. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. The encoded protein is structurally similar to one of the histone-like TAFs, TAF5. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Antigen Description
Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L is an enzyme that in humans is encoded by the TAF5L gene. The product of this gene belongs to the WD-repeat TAF5 family of proteins. This gene encodes a protein that is a component of the PCAF histone acetylase complex. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. The encoded protein is structurally similar to one of the histone-like TAFs, TAF5. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. The function about TAF5L antigen include contributes_to histone acetyltransferase activity; sequence-specific DNA binding transcription factor activity; transcription coactivator activity.
Pathway
Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem.

Citations


Have you cited DCABH-5781 in a publication? Let us know and earn a reward for your research.

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


Bond isomerism, formation of heterocyclic cationic complex, and insertion into the tantalum-fluorine bond in the reaction of TaF5 with MeNCO in CH2Cl2

RUSSIAN JOURNAL OF INORGANIC CHEMISTRY

Authors: Il'in, E. G.; Ovchinnikova, N. A.; Ignatov, M. E.

The reaction of tantalum pentafluoride with methyl isocyanate in dichloromethane was studied by F-19 NMR and IR spectroscopy. The reaction was found to give bond isomers TaF5L, in which methyl isocyanate (L) is coordinated in the monodentate mode via N or O atom, and the heterocyclic cation [(mu-F)(mu-MeNCO)(TaF4)(2)] (n) (+) (n=1,2). On storage of the reaction mixture, a signal in the C-F bond region appears in the spectrum and other new signals appear, which were assigned to the formation of O- and N- bonded isomers of pentafluoro complex of tantalum with aminocarboxylic acid fluoride anions [FC(O)N(Me)TaF5](-) and [N{Me)C(F)OTaF5](-), which can be considered as insertion of MeNCO into the Ta-F bond. The IR spectroscopy data support these assumptions.

Parent and offspring genotypes influence gene expression in early life

MOLECULAR ECOLOGY

Authors: Newhouse, Daniel J.; Barcelo-Serra, Margarida; Tuttle, Elaina M.; Gonser, Rusty A.; Balakrishnan, Christopher N.

Parents can have profound effects on offspring fitness. Little, however, is known about the mechanisms through which parental genetic variation influences offspring physiology in natural systems. White-throated sparrows (Zonotrichia albicollis, WTSP) exist in two genetic morphs, tan and white, controlled by a large polymorphic supergene. Morphs mate disassortatively, resulting in two pair types: tan male x white female (T x W) pairs, which provide biparental care and white male x tan female (W x T) pairs, which provide female-biased care. To investigate how parental composition impacts offspring, we performed RNA-seq on whole blood of WTSP nestlings sampled from nests of both pair types. Parental pair type had a large effect on nestling gene expression, with 881 genes differentially expressed (DE) and seven correlated gene coexpression modules. The DE genes and modules expressed at higher levels in W x T nests with female-biased parental care function in metabolism and stress-related pathways resulting from the overrepresentation of proteolysis and stress-response genes (e.g., SOD2, NR3C1). These results show that parental genotypes and/or associated behaviours influence nestling physiology, and highlight avenues of further research investigating the ultimate implications for the maintenance of this polymorphism. Nestlings also exhibited morph-specific gene expression, with 92 differentially expressed genes, comprising immunity genes and genes encompassed by the supergene. Remarkably, we identified the same regulatory hub genes in these blood-derived expression networks as were previously identified in adult WTSP brains (EPM2A, BPNT1, TAF5L). These hub genes were located within the supergene, highlighting the importance of this gene complex in structuring regulatory networks across diverse tissues.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket