Anti-Radial Nerve Cord Extract monoclonal antibody (DMAB9345)
Mouse anti-Holothuria Radial Nerve Cord Extract monoclonal antibody for WB, IHC
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Radial nerve cord extract from sea cucumber Holothuria glaberrima (Echinodermata: Holothuroidea).
Radial nerve cord extract
Many metazoan neuropeptides are amidated at their C-terminal end and this amidation appears to be critical for the biological activity of the neuropeptide. The only enzyme known to catalyse this important posttranslational modification is peptidylglycine α-amidating monooxygenase (PAM). PAM is a bifunctional enzyme made of two subunits: the peptidylglycine α-hydroxylating monooxygenase (PHM), requiring copper for its action, and the peptidyl- α-hydroxyglycine α-amidating lyase (PAL). In this study, we investigated the presence PAM-like enzymatic activity in an echinoderm. Extracts from radial nerve cords of the sea star Asterias rubens were incubated in vitro with fluorescent peptide substrata in the presence or absence of Cu ++. The results demonstrate a Cu++ -dependent amidation activity similar to the PAM action in other metazoans.
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Hu, B; Yip, HK; et al. Localization of p75 neurotrophin receptor in the retina of the adult SD rat: An immunocytochemical study at light and electron microscopic levels. GLIA 24:187-197(1998).
Kamei, Y; Inagaki, N; et al. Visualization of mitotic radial glial lineage cells in the developing rat brain by cdc2 kinase-phosphorylated vimentin. GLIA 23:191-199(1998).
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