General Notes
This product (Catalog No. DPABH-25436) is a rabbit-derived polyclonal antibody raised against human SMS, targeting the amino acid region 1–366 of the protein. It has been affinity purified by Protein A. Spermine synthase (SMS; EC 2.5.1.22) is a highly specific aminopropyltransferase enzyme that catalyzes the terminal step in the polyamine biosynthetic pathway by transferring an aminopropyl group from decarboxylated S-adenosylmethionine (dcAdoMet) to spermidine, thereby producing spermine and 5′-methylthioadenosine (MTA) as a byproduct. This reaction is essential for maintaining polyamine homeostasis in mammals, where spermine plays critical roles in stabilizing DNA and RNA, modulating ion channels such as NMDA, AMPA, and inward rectifier potassium (Kir) channels, regulating gene expression, and facilitating cellular signaling processes. Found across eukaryotes including animals, plants, and some fungi, as well as certain archaea and bacteria, SMS exhibits evolutionary divergence, with independent origins in different lineages, and its activity is ubiquitous in mammalian tissues but primarily regulated by substrate availability rather than transcriptional control.
DPABH-25436 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-25436 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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