Rat anti-Mouse GINS4 monoclonal antibody for WB, IHC, ICC
GINS4 (GINS Complex Subunit 4) is a Protein Coding gene. Among its related pathways are Cell Cycle, Mitotic and CDK-mediated phosphorylation and removal of Cdc6. GO annotations related to this gene include 3-5 DNA helicase activity. The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al. , 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.
SLD5 or GINS4 is a member of the GINS complex which is involved in initiation of replication and unwinding of DNA. It is highly conserved amongst eukaryotes. The GINS complex in yeast associates with DNA polymerase epsilon and stimulates its catalytic activity. It also interacts with Cdc45 and the MCM2-7 complex to activate the eukaryotic minichromosome maintenance helicase. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. Suitable for use in Western blot, Immunohistochemistry and Immunocytochemistry. Each laboratory shoulddetermine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. The function about GINS4 antigen include protein binding.
Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; DNA strand elongation, organism-specific biosystem; S Phase, organism-specific biosystem; Synthesis of DNA, organism-specific biosystem; Unwinding of DNA, orga.
Yamamoto, F; Yamamoto, M; et al. Identification of Genes That Exhibit Changes in Expression on the 8p Chromosomal Arm by the Systematic Multiplex RT-PCR (SM RT-PCR) and DNA Microarray Hybridization Methods. GENE EXPRESSION 14:217-227(2008).
Ryu, B; Kim, DS; et al. Comprehensive Expression Profiling of Tumor Cell Lines Identifies Molecular Signatures of Melanoma Progression. PLOS ONE 2:-(2007).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.