Anti-SETDB1 monoclonal antibody, clone 6I7B23

Antibody Isotype

IgG1

Clone

6I7B23

Species Reactivity

Mouse; Human; Monkey

Immunogen

Purified recombinant fragment of human SETDB1 expressed in E. Coli.

Conjugate

Application Notes

WB, ELISA, ICC/IF
Recommended dilution:
Western Blot 1:500 - 1:2000
ELISA 1:10000
Immunocytochemistry/Immunofluorescence 1:200 - 1:1000

Alternative Names

SETDB1; SET domain, bifurcated 1; histone-lysine N-methyltransferase SETDB1; ESET; KG1T; KIAA0067; KMT1E; H3-K9-HMTase 4; lysine N-methyltransferase 1E; histone H3-K9 methyltransferase 4; ERG-associated protein with SET domain; ERG-associated protein with

Entrez Gene ID

UniProt ID

Antigen Description

Histone methyltransferase that specifically trimethylates Lys-9 of histone H3. H3 Lys-9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 Lys-9 trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone Lys-9 trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.

Pathway

Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Noncanonical Wnt signaling pathway, organism-specific biosystem;