General Notes
This product (Catalog No. DPABH-14983) is a rabbit-derived polyclonal antibody raised against human SELE, targeting the amino acid region 22–371 of the protein. It has been affinity purified by Protein A. E-selectin, also known as CD62E or endothelial-leukocyte adhesion molecule 1 (ELAM-1), is a calcium-dependent type I transmembrane glycoprotein that belongs to the selectin family of cell adhesion molecules, which also includes P-selectin and L-selectin. It is exclusively expressed on the surface of endothelial cells and plays a critical role in the initial tethering and rolling of leukocytes along the vascular endothelium during the inflammatory response. Structurally, E-selectin consists of an N-terminal C-type lectin domain responsible for carbohydrate recognition, an epidermal growth factor (EGF)-like domain, six short consensus repeat (SCR) domains homologous to complement regulatory proteins, a transmembrane domain, and a short cytoplasmic tail. Its expression is tightly regulated and inducible, primarily triggered by proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), which activate transcription factors like NF-κB, leading to rapid upregulation on endothelial cells within 2–4 hours, peaking at around 4 hours and declining thereafter. While generally absent on resting endothelium, low constitutive expression occurs in specific sites like bone marrow and skin microvascular endothelium.
DPABH-14983 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-14983 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
COA
Publication 
Loading ......
