Rabbit Anti-Human SCGN Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

Recombinant fusion protein of human SCGN (NP_008929.2).

Conjugate

Application Notes

WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200

General Notes

This product (Catalog No. DPABH-02126) is a rabbit-derived IgG polyclonal antibody raised against human SCGN. This antibody exhibits cross-reactivity with SCGN from mouse and rat. It has been affinity purified by protein A. Secretagogin is a highly conserved hexa-EF-hand calcium-binding protein encoded by the SCGN gene, functioning primarily as a calcium sensor that modulates regulated exocytosis in endocrine and neuronal cells. It was discovered in 1998 through screening for antigens specific to human insulinomas and pancreatic beta cells, revealing its role in facilitating insulin secretion. Structurally, secretagogin features three globular domains, each with paired EF-hand motifs that undergo cooperative conformational changes upon binding calcium ions with low affinity (half-maximal ~25 μM), enabling it to sense elevated intracellular calcium levels rather than buffer them.
DPABH-02126 is suitable for use in Western blot (WB) (1:500–1:2,000), IHC (1:50–1:200), IF (immunofluorescence) (1:50–1:200) applications.
DPABH-02126 is supplied as a liquid formulation in PBS with 0.02% sodium azide, 50% glycerol, pH7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

SCGN; CALBL; DJ501N12.8; SECRET; SEGN; setagin

Entrez Gene ID

UniProt ID