Anti-S100A12 monoclonal antibody (DCABH-659)

Mouse anti-Human S100A12 monoclonal antibody for WB, IHC-P, FC Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
2E2
Species Reactivity
Human
Immunogen
Recombinant full length Human S100A12 produced in HEK293T cells (NP_005612).
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/500; IHC-P: 1/150; Flow Cyt: 1/100;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
S100A12; S100 calcium binding protein A12; S100 calcium binding protein A12 (calgranulin C) , S100 calcium binding protein A12 (calgranulin C); protein S100-A12; CAAF1; CAGC
Entrez Gene ID
UniProt ID

Product Background


Gene summary
S100A12 (S100 Calcium Binding Protein A12) is a Protein Coding gene. Diseases associated with S100A12 include kawasaki disease and moorens ulcer. Among its related pathways are Immune System and Blood-Brain Barrier and Immune Cell Transmigration: Pathways Overview. GO annotations related to this gene include calcium ion binding and RAGE receptor binding. An important paralog of this gene is S100A6. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity.
Antigen Description
Calcitermin possesses antifungal activity against C. albicans and is also active against E. coli and P. aeruginosa but not L. monocytogenes and S. aureus. Binds calcium, zinc and copper. Presence of zinc increases the affinity for calcium. Plays an important role in the inflammatory response. Interaction with AGER on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene.
Pathway
Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem.

Citations


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References


Klingelhofer, J; Senolt, L; et al. Up-regulation of metastasis-promoting S100A4 (Mts-1) in rheumatoid arthritis - Putative involvement in the pathogenesis of rheumatoid arthritis. ARTHRITIS AND RHEUMATISM 56:779-789(2007).
Srikrishna, G; Nayak, J; et al. Carboxylated N-Glycans on RAGE Promote S100A12 Binding and Signaling. JOURNAL OF CELLULAR BIOCHEMISTRY 110:645-659(2010).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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