Anti-RSV Monoclonal antibody, Clone 6

The rhizome of Dryopteris crassirhizoma Nakai is an important traditional Chinese medicine listed in Chinese Pharmacopoeia for the treatment of parasitic infestation and viral diseases. In this work, ten enantiomeric pairs of new acylphloroglucinol meroterpenoids, ( +/- )-dryocrassoids A - J [( +/- )-1 - 101, along with six known acylphloroglucinols (11-16) were isolated from the rhizomes of D. crassirhizoma. Notably, these new enantiomers were the first examples of meroterpenoids constructed by acylphloroglucinols and nerolidol-type sesquiterpenes, while ( +/- )-1a/1b, ( +/- )-2a/2b, ( +/- )-4a/4b were obtained as the inseparable interconverting mixtures of keto and enol tautomers. The new structures and absolute configurations were identified by the interpretation of high resolution electron spray ionization mass spectrum (HRESIMS), nuclear magnetic resonance (NMR) spectroscopic data, and quantum chemical electronic circular dichroism (ECD) calculations. In addition, these compounds were assayed for the in vitro antiviral activities against herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), and respiratory syncytial virus (RSV). The results indicated that most of the compounds showed anti-HSV-1 and anti-RSV effects to different extent under the concentration of 100 mu M. This study provided evidences for further study and utilization of D. crassirhizoma in medicinal products.

Introduction: The objective was to estimate the effectiveness of inactivated trivalent vaccine (VE) in preventing hospital flu care (HFC) in Guadalajara, Castile-La Mancha (CLM), Spain, 2018-19 season. Material and methods: Retrospective cohort study (40/2018 to 13/2019 weeks). Sources: Microbiology programme; electronic medical history; population census (INE, 1/7/2018). Cases: Population requiring HFC (hospital emergencies and/or emergency observation unit and/or hospital admissions), confirmed by antigenic test and/or PCR. Preventive fractions [PFv(vaccinated) and PFp(population)] and Necessary number of patients to be vaccinated (NNV) were calculated. Results: 228 HFT occurred [cumulative incidence rate (IR) = 8.9/10(4); >= 65 years = 65%; vaccination coverage = 13% (>= 65 years = 58%); mortality = 9%); maximum incidence in the 6th week (IR = 1.7/10(4)) (in CLM, in 4th)]. Highest peak of RSV occurred in the 3rd (in CLM, in the 52th). PFv (14-65 years) was 96% (PFp = 58%) and in >= 65, 32% (PFp = 21%). NNV = 414. As in Spain, influenza virus A predominated, with A(H3N2) being 13% more prevalent (strain not included in the vaccine). Conclusions: The season was delayed by sustained VRS circulation. The VE was lower than the national one. It is be essential to promote future campaigns to improve vaccination coverage. (C) 2020 Elsevier Espana, S.L.U. All rights reserved.