Sheep Anti-Bovine Red Blood Cells Heterohybridoma [HB5] (CSC-H0146)

This hybridoma produces mAbs (Antibody not secreted) against bovine red blood cells Datasheet

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General Information


Immunogen
Bovine red blood cells
Fusion Species
Mouse X Sheep Heterohybridoma
Immunological Donor
Sheep lymph node
Myeloma
NS1
Clone
HB5
Cell Line Description
This HAT sensitive line can be used as a fusion partner in the production of heterohybridomas.
Application Notes
Fusion partner
Growth Properties
Suspension

Culture Method


Complete Growth Medium
RPMI 1640 with 2 mM glutamine, 1mM Sodium Pyruvate (NaP), supplemented with 10%(v/v) Horse Serum (HS)
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere and maintain cultures between 2-6x10^5 cells/ml
Storage
Liquid nitrogen vapor phase.

Target


Introduction
Red blood cells, or erythrocytes, are the most common type of blood cell and the vertebrate organisms principal means of delivering oxygen (O2) to the body tissues via the blood flow through the circulatory system. [1] They take up oxygen in the lungs or gills and release it while squeezing through the bodys capillaries. These cells cytoplasm is rich in haemoglobin, an iron-containing biomolecule that can bind oxygen and is responsible for the bloods red color.

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