Rabbit Anti-Human RAN (C-terminal) Polyclonal antibody

Name: Rabbit Anti-Human RAN (C-terminal) Polyclonal antibody
SKU: DPABH-15517
Rating: 5 (1 reviews)

Rabbit Anti-Human RAN (C-terminal) Polyclonal antibody for WB, IHC, IF/ICC

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COA

SDS

Datasheet

Key features and details

Specificity: RAN Antibody detects endogenous levels of total RAN.
Host: Rabbit
Application: WB, IHC, IF/ICC
Species Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

A synthesized peptide derived from human RAN, corresponding to a region within C-terminal amino acids.

Conjugate

Unconjugated

Applications

Application Notes

WB 1:500-1:2000
IHC 1:50-1:200
IF/ICC 1:100-1:500

General Notes

This product (Catalog No. DPABH-15517) is a rabbit-derived polyclonal antibody raised against human RAN. This antibody exhibits cross-reactivity with RAN from mouse and rat. It has been affinity purified by Protein A. RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008].
DPABH-15517 is suitable for use in WB 1 (500-1:2000), IHC 1 (50-1:200), IF/ICC 1 (100-1:500) applications.
DPABH-15517 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Entrez Gene ID

5901

UniProt ID

P62826

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Changes in water fluxes partition related to the replacement of native dry forests by crops in the Dry Chaco

JOURNAL OF ARID ENVIRONMENTS

Authors: Rodriguez, Paula; Gimenez, Raul; Nosetto, Marcelo D.; Jobbagy, Esteban G.; Magliano, Patricio N.

Abstract

Land-use change has been the strongest driver of vegetation cover change in the world. In the South American Dry Chaco (similar to 1 M km(2); PPT/ET0 < 0.65), native dry forests are experiencing high deforestation rates that tend to continue in the coming years. Here, we used a hydrological model (Hydrus 1-D) to analyze changes in the water balance triggered by the replacement of dry forests by annual rainfed summer crops. We found that transpiration represented the main water vapour flux in both vegetation covers (59 and 48% of annual rainfall in dry forest and cropland, respectively), followed by evaporation (31 and 30%, respectively) and interception (11 and 9%, respectively). Runoff was five times higher in the cropland compared to the dry forest (10 vs. 2%; p < 0.01). Deep drainage was a negligible water flux in dry forests but it represented 4% of annual rainfall in croplands (p < 0.01), reaching values of zero and 130 mm year(-1) for dry and wet years, respectively. Our results highlight the key role of vegetation cover controlling ecosystem water fluxes in the Dry Chaco. More generally, we suggest that the expansion of dryland cultivation can modify the regional water balance and threaten the primary production of ecosystems in the long run.

Compact formulations for multi-depot routing problems: Theoretical and computational comparisons

COMPUTERS & OPERATIONS RESEARCH

Authors: Bektas, Tolga; Gouveia, Luis; Santos, Daniel

Abstract

Multi-depot routing problems mainly arise in distribution logistics where a fleet of vehicles are used to serve clients from a number of (potential) depots. The problem concerns deciding on the routes of each vehicle and the depots from which the vehicles depart, so as to minimize the total cost of travel. This paper reviews a number of existing compact formulations, and proposes new ones, for two types of multi-depot routing problems, one that includes the depot selection decisions, and the other where depots are pre-selected. The formulations are compared theoretically in terms of the strength of their linear programming relaxation, and computationally in terms of the running time needed to solve the instances to optimality. (c) 2020 Elsevier Ltd. All rights reserved.