Mouse Anti-Radial glia and Neural progenitor cells Hybridoma [SD2] (CSC-H1480)

This hybridoma produces mAbs against Radial glia and neural progenitor cells

General Information


Immunogen
Radial glia and neural progenitor cells
Fusion Species
Mouse x Mouse Hybridoma
Immunological Donor
Mouse spleen
Clone
SD2
Cell Line Description
This cell line produces a monoclonal antibody RC1 (IgG class). Immunochistochemically, RC1 antibody reacts with radial glia and neural progenitor cells in CNS.
Application Notes
N/A
Morphology
round

Culture Method


Complete Growth Medium
RPMI-1640, supplemented with 15%(v/v) FBS
Storage
Liquid nitrogen vapor phase.

Target


Introduction
Radial glial cells are a pivotal cell type in the developing central nervous system (CNS) involved in key developmental processes, from patterning and neuronal migration to their recently discovered role as precursors during neurogenesis. They arise early in development from neuroepithelial cells. Radial phenotype is typically transient, but some cells, such as Bergmann glia in the cerebellum and Muller glia in the retina, retain radial glia-like morphology postnatally. According to recent research, during the late stages of cortical development, radial glial cells divide asymmetrically in the ventricular zone to generate radial glial cells, postmitotic neurons and intermediate progenitor cells. Intermediate progenitor cells then divide symmetrically in the subventricular Neural progenitor cells are more promising candidates for replacing damaged and degenerated neurons because they are self-renewing, which allows for the in vitro production of many cells with minimal donor material. In order to confirm that the new neurons formed from neural progenitor cells are a part of a functional network, the presence of synapse formation is required. A study by Ma, Fitzgerald et al. is the first demonstration of murine neural stem and progenitor cell-derived functional synapse and neuronal network formation on a 3D collagen matrix. The neural progenitor cells expanded and spontaneously differentiated into excitable neurons and formed synapses; furthermore, they retained the ability to differenitate into the three neural tissue lineages. It was also demonstrated that not only active synaptic vesicle recycling occurred, but also that excitatory and inhibitory connections capable of generating action potentials spontaneously were formed. Thus, neural progenitor cells are a viable and relatively unlimited source for creating functional neurons.

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