Anti-RING1 monoclonal antibody (DCABH-6084) Knockout Validated

Mouse anti-Human RING1 monoclonal antibody for WB, ELISA, FC, IHC-P

Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
9D23G5
Species Reactivity
Human
Immunogen
Recombinant fragment corresponding to Human RING1 aa 79-263.Database link: Q06587
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/500 - 1/2000; ELISA: 1/10000; Flow Cyt: 1/200 - 1/400; IHC-P: 1/200 - 1/1000;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
RING1; ring finger protein 1; E3 ubiquitin-protein ligase RING1; RNF1; polycomb complex protein RING1; really interesting new gene 1 protein
Entrez Gene ID
UniProt ID

Product Background


Gene summary
RING1 (Ring Finger Protein 1) is a Protein Coding gene. Diseases associated with RING1 include occult macular dystrophy and macular degeneration, age-related, 1. Among its related pathways are Transport to the Golgi and subsequent modification and SUMOylation. GO annotations related to this gene include chromatin binding and ubiquitin-protein transferase activity. An important paralog of this gene is RNF2. This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4.
Antigen Description
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of Lys-119 of histone H2A, thereby playing a central role in histone code and gene regulation. H2A Lys-119 ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase RING1 is an enzyme that in humans is encoded by the RING1 gene. This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. The function about RING1 antigen include chromatin binding; ligase activity; metal ion binding; protein binding; ubiquitin-protein ligase activity; zinc ion binding.
Pathway
Delta-Notch Signaling Pathway, organism-specific biosystem.

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References


Cmarko, D; Verschure, PJ; et al. Polycomb group gene silencing proteins are concentrated in the perichromatin compartment of the mammalian nucleus. JOURNAL OF CELL SCIENCE 116:335-343(2003).
Saurin, AJ; Shiels, C; et al. The human polycomb group complex associates with pericentromeric heterochromatin to form a novel nuclear domain. JOURNAL OF CELL BIOLOGY 142:887-898(1998).

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