Rabbit Anti-RELA Polyclonal Antibody (DPABH-22935)

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse
Immunogen
A synthetic peptide of human RELA
Conjugate
Unconjugated

Applications


Application Notes
WB:    1:500 - 1:1000; IHC:    1:20 - 1:50; IF:    1:20 - 1:50; FC:    1:20 - 1:50
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Western blot analysis of extracts of various cell lines, using RELA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Target


Alternative Names
RELA; v-rel avian reticuloendotheliosis viral oncogene homolog A; p65; NFKB3; transcription factor p65; NF-kappa-B p65delta3
Entrez Gene ID
UniProt ID

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Ivanov, VN; NikolicZugic, J; et al. Transcription factor activation during signal-induced apoptosis of immature CD4(+)CD8(+) thymocytes - A protective role of c-Fos. JOURNAL OF BIOLOGICAL CHEMISTRY 272:8558-8566(1997).
Wang, WH; Palumbo, AM; et al. Identification of p65-Associated Phosphoproteins by Mass Spectrometry after On-Plate Phosphopeptide Enrichment Using Polymer-oxotitanium Films. JOURNAL OF PROTEOME RESEARCH 9:3005-3015(2010).

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