GeneralInformation
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with SRBC. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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Valentijn, TM; Hoeks, SE; et al. The Impact of Perioperative Red Blood Cell Transfusions on Postoperative Outcomes in Vascular Surgery Patients. ANNALS OF VASCULAR SURGERY 29:-(2015).
Song, H; Hashash, YMA; et al. Characterization of stress-strain behaviour of red blood cells (RBCs), part II: response of malaria-infected RBCs. INVERSE PROBLEMS IN SCIENCE AND ENGINEERING 23:413-424(2015).
COA
Certificate of Analysis
