Anti-RAG2 monoclonal antibody, clone 5E6

Clone

5E6

Species Reactivity

Human

Immunogen

Recombinant protein corresponding to amino acids 350-527 of human RAG2.

Conjugate

Application Notes

ELISA (1:10000); WB (1:500-1:2000) & The optimal working dilution should be determined by the end user.

Alternative Names

RAG2; recombination activating gene 2; V(D)J recombination-activating protein 2; RAG-2;

Entrez Gene ID

UniProt ID

Antigen Description

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Pathway

C-MYB transcription factor network, organism-specific biosystem; Primary immunodeficiency, organism-specific biosystem; Primary immunodeficiency, conserved biosystem;