Anti-RAD9A monoclonal antibody, clone 8C22

Antibody Isotype

IgG2a

Clone

8C22

Species Reactivity

Human

Immunogen

Protein expressed in 293T cells transfected with Human Rad9 expression vector

Conjugate

Application Notes

WB: 1/2000; IHC-P: 1/50;

Alternative Names

RAD9A; RAD9 homolog A (S. pombe); RAD9, RAD9 (S. pombe) homolog; cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A; RAD9;

Entrez Gene ID

UniProt ID

Antigen Description

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3-> 5 double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Pathway

Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; G2/M Checkpoints, organism-specific biosystem; Regulation of Telomerase, organism-specific biosystem;