Human Anti-Bacteria Pseudomonas aeruginosa Lipopolysaccharide (LPS) of Fisher Immunotype 7 Hybridoma [9F8] (CSC-H1066)

This hybridoma produces mAbs (IgM) against Bacteria Pseudomonas aeruginosa Lipopolysaccharide (LPS) of fisher Immunotype 7 Datasheet

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General Information


Isotype
IgM
Fusion Species
Human X Human Hybridoma
Immunological Donor
Human
Clone
9F8
Cell Line Description
B cells obtained from a patient with cystic fibrosis (known to have a chronic infection with P. aeruginosa) were transformed with Epstein-Barr virus (EBV). The antibody is specific for the lipopolysaccharide of the Fisher immunotype 7 of P. aeruginosa.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
IMDM with 2 mM L-glutamine
, supplemented with 15% heat-inactivated FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 3-4 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.

Target


Introduction
Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacteria are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients. P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42 centigrade. P. aeruginosa is capable of growth in diesel and jet fuel, where it is known as hydrocarbon utilizing microorganisms (or "HUM bugs"), causing microbial corrosion. It creates dark gellish mats sometimes improperly called "algae".

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