Anti-PRKACA monoclonal antibody (CAB-4312RH)

Rabbit anti-PRKACA monoclonal antibody for WB, IP, IHC Datasheet

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Specifications


Host Species
Rabbit
Clone
FQ3203Z
Species Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues near the C-terminus of human PRKACA was used as immunogen.
Conjugate
Unconjugated

Applications


Application Notes
WB: 1:100000-200000; IHC: 1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
PRKACA; protein kinase, cAMP-dependent, catalytic, alpha; cAMP-dependent protein kinase catalytic subunit alpha; PKACa; PKA C-alpha; protein kinase A catalytic subunit
Entrez Gene ID
UniProt ID

Product Background


Gene summary
PRKACA (Protein Kinase CAMP-Activated Catalytic Subunit Alpha) is a Protein Coding gene. Diseases associated with PRKACA include pigmented nodular adrenocortical disease, primary, 4 and hepatocellular fibrolamellar carcinoma. Among its related pathways are Platelet activation, signaling and aggregation and Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKG1. This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Antigen Description
PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription. PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunit (RI and RII) and three C subunit isoforms (C-alpha, C-beta, and C-gamma) have been identified each possessing distinct cAMP binding properties and resulting in different phosphorylation states. C subunit is activated through autophosphorylation and direct phosphorylation at Thr197 by PDK-1.Defective regulation of PKA holoenzyme activity has been linked to the progression of cardiovascular disease, certain endocrine disorders and cancers. 0The function about PRKACA antigen include ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding.
Pathway
Adaptive Immune System, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem.

Citations


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References


Cui, C; Zhao, HM; et al. CDC25B Acts as a Potential Target of PRKACA in Fertilized Mouse Eggs. BIOLOGY OF REPRODUCTION 79:991-998(2008).

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