Anti-PCNT monoclonal antibody (DCABH-8442)

Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
nBcdbn 39255
Species Reactivity
Human, Chinese Hamster
Immunogen
The pericentrin clone used is 1.7 kb in size and is derived from within residues 100-600 of mouse pericentrin 1. It was expressed as a fusion protein. The corresponding amino acids are present in both pericentrin and kendrin (pericentrin-2) so this antibo
Conjugate
Unconjugated

Applications


Application Notes
ICC/IF: 1 μg/ml; Flow Cyt: 1μg/106 cells.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
PCNT; pericentrin; PCNT2, pericentrin 2 (kendrin); KEN; kendrin; KIAA0402
Entrez Gene ID
UniProt ID

Product Background


Gene summary
PCNT (Pericentrin) is a Protein Coding gene. Diseases associated with PCNT include microcephalic osteodysplastic primordial dwarfism, type ii and seckel syndrome 4. Among its related pathways are Cell Cycle, Mitotic and Organelle biogenesis and maintenance. GO annotations related to this gene include calmodulin binding. An important paralog of this gene is AKAP9. The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene.
Antigen Description
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. This protein localizes to the centrosome and recruits proteins to the pericentriolar matrix(PCM) to ensure proper centrosome and mitotic spindle formation, and thus, uninterrupted cell cycle progression. This gene is implicated in many diseases and disorders, including congenital disorders such as microcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel syndrome. The protein encoded by this gene is expressed in the cytoplasm and centrosome throughout the cell cycle, and to a lesser extent, in the nucleus. This ensures normal function and organization of the centrosomes, mitotic spindles, and cytoskeleton, and by extension, regulation over cell cycle progression and checkpoints. Downregulation of PCNT disrupted mitotic checkpoints and arrested the cell at the G2/M checkpoint, leading to cell death. Moreover, microtubule functioning was also disrupted, resulting in mono- or multipolar spindles, chromosomal misalignment, premature sister chromatid separation, and aneuploidy.
Pathway
Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem; Loss of proteins required for interphase microtubule organization??from the centrosome, organism-specific biosystem; Mitotic G2-G2/M phases, organism-specific biosystem.

Citations


Have you cited DCABH-8442 in a publication? Let us know and earn a reward for your research.

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Mouse IgG1 Isotype Control DAGIC1206 FC ELISA ICC IHC Mouse PDF Inquiry

References


Evaluation of Mechanical Properties in Nanocomposites Containing Carbon Nanotubes Below and Above Percolation Threshold

JOM

Authors: Zare, Yasser; Rhee, Kyong Yop

In this paper, several models are introduced for tensile modulus and strength of polymer nanocomposites containing dispersed and networked carbon nanotubes (CNT) below and above percolation threshold. The model predictions are compared in similar conditions to determine the role of nanoparticle structure in the mechanical properties of polymer/CNT nanocomposites (PCNT). The predictions are also compared with the experimental data of several samples to offer the suitable models for the mechanical properties of PCNT. Lastly, the influences of the main parameters on the tensile modulus and strength of PCNT are studied. The networked nanoparticles cause higher levels of modulus and strength compared to the dispersed nanoparticles. The high level of the CNT aspect ratio causes a small percolation threshold in PCNT, but the Ouali model shows the ineffective role of this parameter in their predictions.

Multi-walled carbon nanotubes photochemistry: A mechanistic view of the effect of impurities and oxygen-containing surface groups

CARBON

Authors: Rodriguez Sartori, Damian; Laura Dell'Arciprete, Maria; Magnacca, Giuliana; Calza, Paola; Laurenti, Enzo; Gonzalez, Monica C.

Continuous photolysis experiments and transient absorption spectroscopy were performed in combination with other techniques including HRTEM, XPS, Raman, TGA, and ESR spectroscopy, to investigate the role of residual metals and amorphous carbon on the photochemical process taking place after 350-355 nm light irradiation of as obtained commercial multi-walled carbon nanotubes, denoted as pCNT. The results indicate that 350-355 nm photolysis of pCNT leads to the oxidation of surface oxygen-containing groups and defects which in turn are eliminated leading to more graphitic -like multi-walled carbon nanotubes (MWCNT). Residual metal catalysts and oxygen containing amorphous carbon and oxidized C-functionalities of MWCNT play an important role in the generation of MWCNT photoinduced charge-separated states. The process of 350 nm excitation of pCNT leads to exciton formation followed by hole transfer to metal oxides and further oxidation of C-O functionalities. A plausible mechanism explaining the elimination of oxidized groups attached to pCNT graphene walls and amorphous carbon and leading to more graphite-like CNTs is discussed. The results presented may have implications in the nanoscale semiconductor materials for optoelectronics applications. (C) 2018 Elsevier Ltd. All rights reserved.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:
Verification code
Click image to refresh the verification code.

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket