Anti-PAGE5 polyclonal antibody (DPABH-08937)

Rabbit anti-Human PAGE5 (aa 109-130) polyclonal antibody for WB, IHC-P

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Synthetic peptide within Human PAGE5 aa 109-130 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.Database link: Q96GU1
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/100 - 1/500; IHC-P: 1/10 - 1/50;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
PAGE5; P antigen family, member 5 (prostate associated); CT16; CT16.1; CT16.2; GAGEE1
Entrez Gene ID
UniProt ID

Product Background


Gene summary
PAGE5 (PAGE Family Member 5) is a Protein Coding gene. An important paralog of this gene is PAGE3. This gene is a member of family of proteins that are expressed in a variety of tumors and in some fetal and reproductive tissues. The encoded protein may protect cells from programmed cell death. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found.
Antigen Description
This gene is a member of the GAGE family, which is expressed in a variety of tumors and in some fetal and reproductive tissues. The protein encoded by this gene shares a sequence similarity with other GAGE/PAGE proteins. It may also belong to a family of CT (cancer-testis) antigens. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants have not been determined. Protein Symbol:Q96GU1-PAGE5_HUMANRecommended name:P antigen family member 5Protein Accession:Q96GU1Secondary Accessions:Q2NL97Q5JUL0Q8WWL9.

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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


An intraresidual i(HCA)CO(CA)NH experiment for the assignment of main-chain resonances in N-15, C-13 labeled proteins

JOURNAL OF BIOMOLECULAR NMR

Authors: Mantylahti, Sampo; Tossavainen, Helena; Hellman, Maarit; Permi, Perttu

An improved pulse sequence, intraresidual i(HCA)CO(CA)NH, is described for establishing solely C-13'(i), N-15(i), H-1(N)(i) connectivities in uniformly N-15/C-13-labeled proteins. In comparison to the "out-and-back" style intra-HN(CA)CO experiment, the new pulse sequence offers at least two-fold higher experimental resolution in the C-13' dimension and on average 1.6 times higher sensitivity especially for residues in alpha-helices. Performance of the new experiment was tested on a small globular protein ubiquitin and an intrinsically unfolded 110-residue cancer/testis antigen CT16/PAGE5. Use of intraresidual i(HCA)CO(CA)NH experiment in combination with the established HNCO experiment was crucial for the assignment of highly disordered CT16.

Melanoma-Associated Cancer-Testis Antigen 16 (CT16) Regulates the Expression of Apoptotic and Antiapoptotic Genes and Promotes Cell Survival

PLOS ONE

Authors: Nylund, Camilla; Rappu, Pekka; Pakula, Eveliina; Heino, Aleksi; Laato, Laura; Elo, Laura L.; Vihinen, Pia; Pyrhonen, Seppo; Owen, Gethin R.; Larjava, Hannu; Kallajoki, Markku; Heino, Jyrki

Cancer-testis (CT) antigens are predominantly expressed in testis or placenta, but absent in most adult tissues. During malignant transformation CT genes are often activated. CT antigen 16 (CT16, PAGE5) is frequently expressed in advanced melanoma but its biological function has been unknown. To examine the role of CT16 in cell survival we knocked it down in A2058 melanoma cells using specific siRNAs and exposed the cells to cancer drug cisplatin known to induce apoptosis. As a result, cell survival was markedly decreased. To study the effects of CT16 on cell survival in more detail, the cellular gene expression profiles were investigated after CT16 silencing in CT16 positive A2058 melanoma cells, as well as after CT16 overexpression in CT16 negative WM-266-4 melanoma cells. Among the 11 genes both upregulated by CT16 silencing and downregulated by CT16 overexpression or vice versa, 4 genes were potentially apoptotic or antiapoptotic genes. CT16 was recognized as a positive regulator of antiapoptotic metallothionein 2A and interleukin 8 genes, whereas it inhibited the expression of apoptosis inducing dickkopf 1 (DKK1) gene. In addition CT16 enhanced the expression of fatty acid binding protein 7, a known promoter of melanoma progression. The effect of CT16 on DKK1 expression was p53 independent. Furthermore, CT16 did not regulate apoptotic genes via DNA methylation. In twenty melanoma metastasis tissue samples average DKK1 mRNA level was shown to be significantly (p<0.05) lower in high CT16 expressing tumors (n = 3) when compared to the tumors with low CT16 expression (n = 17). Thus, our results indicate that CT16 promotes the survival of melanoma cells and is therefore a potential target for future drug development.

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