Anti-OTUB1 monoclonal antibody, clone FQS24138(C)

Antibody Isotype

IgG

Clone

FQS24138(C)

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human OTUB1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.Database link: Q96FW1

Conjugate

Application Notes

WB: 1/1000 - 1/5000;

Alternative Names

OTUB1; OTU domain, ubiquitin aldehyde binding 1; ubiquitin thioesterase OTUB1; FLJ20113; FLJ40710; otubain-1; OTU-domain Ubal-binding 1; deubiquitinating enzyme OTUB1; ubiquitin-specific protease otubain 1; ubiquitin-specific-processing protease OTUB1; OT

Entrez Gene ID

UniProt ID

Antigen Description

Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of Lys-48-linked polyubiquitin chains, but not Lys-63-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to Lys-48-linked ubiquitin.