Anti-OLR1 monoclonal antibody, clone 442320

This study investigated the impact of nine polymorphisms located in the CSN2, CSN3, CSN1S1, CSN1S2, and parity on reproductive traits. The analysis was conducted on 165 Holstein-Friesian cows. Genotypes were identified using PCR-RFLP. The data of reproductive traits for four lactations were evaluated. Statistical analysis was carried out using least squares of the GLM procedures. Results indicated that CSN2 had significant effects on days before first insemination and first insemination to pregnancy interval. The SNP at the CSN3 was significantly associated with gestation length. A novel effect of OLR1-C223A on age at first calving was observed in the present study. Moreover, DGAT1 and LGB markers were significantly associated with calving interval and days before first insemination, respectively. In addition, significant environmental effects were as follows: calving year with days before first oestrus, days open, and first insemination to pregnancy interval; season with days open, first insemination to pregnancy interval, and calving interval; parity with days before first oestrus and days open. The present results and novel associations may therefore be useful and indicative for future studies on a genetic basis of cattle reproduction traits.

This study evaluates the efficiency of domestic wastewater treatment via Sponge-Based Moving Bed Biofilm Reactor (S-MBBR). The laboratory-based treatment plan uses polyurethane sponge with a specific surface area was 260 m(2)/m(3) as a carrier. The treatment plan operated under four different organic load rate: OLR1 = 0.4 kg BOD/m(3).day; OLR2 = 0.6 kg BOD/m(3).day; OLR3 = 0.8 kg BOD/m(3).day; and OLR4 = 1.0 kg BOD/m(3).day. During 80 d of the experiment, the highest treatment efficiency was at the organic load rate of 0.4 kg BOD/m(3).day, with COD, SS, TN and TP were found to be 85.0 +/- 12.9%, 85.7 +/- 5.3%, 68.9 +/- 1.7%, and 40.3 +/- 0.2%, respectively. In which, the influent SS concentration were from 117.3 to 126.0 mg/L, the effluent concentration were in ranged 18.0 to 34.22 mg/L, respectively. The values of influent and effluent COD were 298.8 +/- 12.88 and 44.8 +/- 3.78 mg/L in turn. The OLR1 influent TN, TP concentrations were respectively 47.9 +/- 2.11 and 3.6 +/- 0.15 mg/L; the effluent TN, TP concentration were 14.9 +/- 0.18 and 2.2 +/- 0.06 mg/L, respectively. The study suggests that the effluent is within the allowable limits of National technical regulation on domestic wastewater (Column B1), indicating the applicability of S-MBBR for the domestic wastewater treatment plant.