Anti-NR4A1 monoclonal antibody (DCABH-179)

Rabbit Anti-NR4A1 monoclonal antibody for WB, ICC/IF, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
FU2814-08
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:500-1:1,000; ICC: 1:50-1:200 IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of NUR77 on rat brain cells lysates using anti-NUR77 antibody at 1/500 dilution.
Fig2: ICC staining NUR77 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining NUR77 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining NUR77 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse ovarian using anti-NUR77 antibody. Counter stained with hematoxylin.

Target


Alternative Names
Early response protein NAK1; GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10

Citations


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References


Hamers, AAJ; Vos, M; et al. Bone Marrow-Specific Deficiency of Nuclear Receptor Nur77 Enhances Atherosclerosis. CIRCULATION RESEARCH 110:428-U147(2012).
Tao, R; Hancock, WW; et al. Resistance of Foxp3+Regulatory T Cells to Nur77-Induced Apoptosis Promotes Allograft Survival. PLOS ONE 3:-(2008).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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