Anti-NIT2 monoclonal antibody (DCABH-730)

Mouse anti-Human NIT2 monoclonal antibody for WB, IHC-P Datasheet

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Host Species
Antibody Isotype
Species Reactivity
Recombinant full length Human NIT2 produced in HEK293T cells (NP_064587).


Application Notes
WB: 1/1000; IHC-P: 1/50;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.


Alternative Names
NIT2; nitrilase family, member 2; omega-amidase NIT2; Nit protein 2; nitrilase homolog 2; MGC111199
Entrez Gene ID
UniProt ID

Product Background

Antigen Description
Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle. The function about NIT2 antigen include hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; molecular_function; omega-amidase activity.
Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem.


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Xiao, XD; Marzluf, GA; et al. Identification of the native NIT2 major nitrogen regulatory protein in nuclear extracts of Neurospora crassa. GENETICA 97:153-163(1996).
Grsic-Rausch, S; Kobelt, P; et al. Expression and localization of nitrilase during symptom development of the clubroot disease in Arabidopsis. PLANT PHYSIOLOGY 122:369-378(2000).

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