Anti-NGDN polyclonal antibody (DPABH-15139)

Rabbit anti-Human NGDN (aa 61-209) polyclonal antibody for IHC-P

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Recombinant fragment, corresponding to amino acids 61-209 of Human NGDN (Q8NEJ9).
Conjugate
Unconjugated

Applications


Application Notes
IHC-P: 1/200 - 1/500.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
NGDN; neuroguidin, EIF4E binding protein; NGD; LCP5; CANu1; lpd-2
Entrez Gene ID
UniProt ID

Citations


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References


High expression of neuroguidin increases the sensitivity of acute myeloid leukemia cells to chemotherapeutic drugs

JOURNAL OF HEMATOLOGY & ONCOLOGY

Authors: Chen, Kejun; Lu, Shuqing; Cheng, Hui; Tang, Gusheng; Liu, Min; Zhou, Hong; Wang, Jianmin

Neuroguidin (NGDN) is a eukaryotic translation initiation factor 4E binding protein. The purpose of this study was to clarify the function of NGDN and its possible mechanism of action in human myeloid leukemia cells. Proliferation inhibition and apoptosis in NGDN over-expressing myeloid multidrug-resistant leukemia cells (K562/A02-NGDN) was significantly higher than in control K562/A02 cells following treatment with vincristine, etoposide, and epirubicin, indicating that NGDN over-expression can increase the sensitivity of multidrug-resistant leukemia cells to chemotherapeutic drugs. Furthermore, NGDN knock-down in K562/A02 cells resulted in the activation of multiple tumor-related signaling pathways, especially the mammalian target of rapamycin (mTOR) pathway.

Identification of the RNA polymerase I-RNA interactome

NUCLEIC ACIDS RESEARCH

Authors: Pineiro, David; Stoneley, Mark; Ramakrishna, Manasa; Alexandrova, Jana; Dezi, Veronica; Juke-Jones, Rebekha; Lilley, Kathryn S.; Cain, Kelvin; Willis, Anne E.

Ribosome biogenesis is a complex process orchestrated by a host of ribosome assembly factors. Although it is known that many of the proteins involved in this process have RNA binding activity, the full repertoire of proteins that interact with the precursor ribosomal RNA is currently unknown. To gain a greater understanding of the extent to which RNA-protein interactions have the potential to control ribosome biogenesis, we used RNA affinity isolation coupled with proteomics to measure the changes in RNA-protein interactions that occur when rRNA transcription is blocked. Our analysis identified 211 out of 457 nuclear RNA binding proteins with a >3-fold decrease in RNA-protein interaction after inhibition of RNA polymerase I (RNAPI). We have designated these 211 RNA binding proteins as the RNAPI RNA interactome. As expected, the RNAPI RNA interactome is highly enriched for nucleolar proteins and proteins associated with ribosome biogenesis. Selected proteins from the interactome were shown to be nucleolar in location and to have RNA binding activity that was dependent on RNAPI activity. Furthermore, our data show that two proteins, which are required for rRNA maturation, AATF and NGDN, and which form part of the RNA interactome, both lack canonical RNA binding domains and yet are novel pre-rRNA binding proteins.

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