Anti-NEK6 monoclonal antibody (DCABH-612)

Mouse anti-Human NEK6 monoclonal antibody for WB, IHC-P Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
3I8
Species Reactivity
Human
Immunogen
Recombinant Human NEK6 expressed in 293T cells
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/1000; IHC-P: 1/50;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
NEK6; NIMA (never in mitosis gene a)-related kinase 6; SID6-1512
Entrez Gene ID

Product Background


Gene summary
NEK6 (NIMA Related Kinase 6) is a Protein Coding gene. Among its related pathways are Regulation of Glucokinase by Glucokinase Regulatory Protein and Mitotic Prophase. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is NEK2. The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
Antigen Description
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation. Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Involved in G2/M phase cell cycle arrest induced by DNA damage. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Serine/threonine-protein kinase Nek6 is an enzyme that in humans is encoded by the NEK6 gene. The Aspergillus nidulans never in mitosis A (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. [supplied by OMIM].

Citations


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References


Belham, C; Roig, J; et al. A mitotic cascade of NIMA family kinases - Nercc1/Nek9 activates the Nek6 and Nek7 kinases. JOURNAL OF BIOLOGICAL CHEMISTRY 278:34897-34909(2003).
Hayward, DG; Newbatt, Y; et al. Identification by High-Throughput Screening of Viridin Analogs as Biochemical and Cell-Based Inhibitors of the Cell Cycle-Regulated Nek2 Kinase. JOURNAL OF BIOMOLECULAR SCREENING 15:918-927(2010).

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