General Notes
This product (Catalog No. DPABH-04477) is a rabbit-derived IgG polyclonal antibody raised against human NEK3, targeting the amino acid region 165–265 of the protein. This antibody exhibits cross-reactivity with NEK3 from mouse, rat, pig, cow, horse, and rabbit. It has been affinity purified by Protein A. This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Two functional alleles for this gene have been identified in humans. The reference genome assembly (GRCh38) represents a functional allele that is associated with the inclusion of an additional coding exon in protein-coding transcripts, compared to an alternate functional allele that lacks the exon.
DPABH-04477 is suitable for use in Western blot (WB) (1:500–1:2,000) application.
DPABH-04477 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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