Magic™ Anti-Nucleolin (Phospho T76) monoclonal antibody, clone FQS9191-3

Malignant cutaneous melanoma (CM) is an extremely aggressive cancer characterized by a high level of metastatic activity and unfavorable prognosis due to a high incidence of relapses, as well as resistance to standard chemotherapy. Cutaneous melanoma accounts for 80% of deaths from malignant skin tumors. Nucleolin/C23 and nucleophosmin/B23, which constitute altogether 70% of the nucleolus volume, are promising targets for molecular therapy of melanoma. These proteins perform many important functions in the cell, so disruption of the NCL and/or NPM gene structure and abnormal expression of the C23 and B23 proteins they encode, can lead to unlimited cell proliferation and progression of a tumor. Therefore, investigation of the structure and expression of these genes is a topical problem, which is important for understanding the mechanisms of CM carcinogenesis and for the development of new therapeutic approaches. This paper describes new NCL and NPM polymorphisms, as well as the levels of C23 and B23 expression in normal tissues, CM and mucosal melanoma.

The first 5,10,15,20-tetra-(4-(triazol-1-yl)phenyl) porphyridine complex, [Zn2Cl4 (5,10,-15,20-tetra-(4-(triazol-1-yl) phenyl) porphyridine)](n)nCl center dot nH(3)O center dot 7nH(2)O (1), has been synthesized via solvothermal reactions and characterized by single-crystal X-ray diffraction. Complex 1 is characteristic of a one-dimensional (1-D) structure, consisting of neutral [Zn2Cl4 (5,10,15,20-tetra-(4-(triazol-1-yl)phenyl)porphyridine)](n) chains, isolated chloride ions and lattice water molecules. The zinc ion is in a four-coordinated tetrahedral geometry, and the porphyrin macrocycle is saddle-distorted. Photoluminescence measurement with solid-state samples discovers that it exhibits an emission in the green region of the light spectrum. Time-dependent density functional theory (TDDFT) calculation discovers that this emission can be attributed to the pi-pi* charge transfer. The cyclic voltammetry CV) measurement reveals that it possesses an oxidation peak at 0.37 V.