Anti-NCAM1 monoclonal antibody (CAB-5920RH)

Rabbit Anti-NCAM1 monoclonal antibody for WB, ICC/IF, IHC, IP, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KG2132
Species Reactivity
Human, Zebrafish
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y; Lane 2: Human brain
Fig2: ICC staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
antigen MSK39 identified by monoclonal antibody 5.1H11; antigen recognized by monoclonal antibody 5.1H11; CD56; cell adhesion molecule, neural, 1; MSK 39; MSK39

Citations


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References


Jain, M; Velez, JI; et al. A cooperative interaction between LPHN3 and 11q doubles the risk for ADHD. MOLECULAR PSYCHIATRY 17:741-747(2012).
Lapalombella, R; Kern, H; et al. Persistence of regenerative myogenesis in spite of down-regulation of activity-dependent genes in long-term denervated rat muscle. NEUROLOGICAL RESEARCH 30:197-206(2008).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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