Anti-NCAM1 monoclonal antibody (CAB-5920RH)

Rabbit Anti-NCAM1 monoclonal antibody for WB, ICC/IF, IHC, IP, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KG2132
Species Reactivity
Human, Zebrafish
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y; Lane 2: Human brain
Fig2: ICC staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
antigen MSK39 identified by monoclonal antibody 5.1H11; antigen recognized by monoclonal antibody 5.1H11; CD56; cell adhesion molecule, neural, 1; MSK 39; MSK39

Citations


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References


Rusakiewicz, S; Semeraro, M; et al. Immune Infiltrates Are Prognostic Factors in Localized Gastrointestinal Stromal Tumors. CANCER RESEARCH 73:3499-3510(2013).
Chen, CF; Shiue, YL; et al. Laying traits and underlying transcripts, expressed in the hypothalamus and pituitary gland, that were associated with egg production variability in chickens. THERIOGENOLOGY 68:1305-1315(2007).

Custom Antibody Labeling


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