Anti-MKRN1 monoclonal antibody (DCABH-940)

Mouse anti-Human MKRN1 monoclonal antibody for WB Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2b
Clone
4G0
Species Reactivity
Human
Immunogen
Full length recombinant Human MKRN1 (NP_038474) produced in HEK293T cells.
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/2000;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
MKRN1; makorin ring finger protein 1; E3 ubiquitin-protein ligase makorin-1; RNF61; RING finger protein 61; FLJ21334
Entrez Gene ID
UniProt ID

Product Background


Gene summary
MKRN1 (Makorin Ring Finger Protein 1) is a Protein Coding gene. Diseases associated with MKRN1 include west nile virus. Among its related pathways are Immune System and Antigen processing- Ubiquitination and Proteasome degradation. GO annotations related to this gene include poly(A) RNA binding and ligase activity. An important paralog of this gene is MKRN2. This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antigen Description
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. The function about MKRN1 antigen include chromatin binding; ligase activity; metal ion binding; molecular_function; nucleic acid binding; zinc ion binding.
Pathway
Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem.

Citations


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References


Shimada, H; Shiratori, T; et al. Identification of Makorin 1 as a novel SEREX antigen of esophageal squamous cell carcinoma. BMC CANCER 9:-(2009).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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