Anti-MBD3 monoclonal antibody (DCABH-472)

Mouse anti-Human MBD3 monoclonal antibody for WB, IHC-P Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
217C702
Species Reactivity
Human
Immunogen
Synthetic peptide: CKAFMVTDEDIRKQEE conjugated to KLH, corresponding to amino acids 215-230 of Human MBD3 (NP_003917.1).
Conjugate
Unconjugated

Applications


Application Notes
WB: 2 μg/ml; IHC-P: 10 μg/ml;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
MBD3; methyl-CpG binding domain protein 3
Entrez Gene ID

Product Background


Gene summary
MBD3 (Methyl-CpG Binding Domain Protein 3) is a Protein Coding gene. Diseases associated with MBD3 include subacute cutaneous lupus erythematosus and silver-russell syndrome. Among its related pathways are Gene Expression and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3. GO annotations related to this gene include chromatin binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding. An important paralog of this gene is MBD2. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene.
Antigen Description
Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Methyl-CpG-binding domain protein 3 is a protein that in humans is encoded by the MBD3 gene. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA but instead binds to hydroxymethylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.

Citations


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References


Lorincz, MC; Schubeler, D; et al. Methylation-mediated proviral silencing is associated with MeCP2 recruitment and localized histone H3 deacetylation. MOLECULAR AND CELLULAR BIOLOGY 21:7913-7922(2001).
Uno, T; Nomura, Y; et al. Expression, purification and characterization of methyl DNA binding protein from Bombyx mori. JOURNAL OF INSECT SCIENCE 5:-(2005).

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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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