Rabbit Anti-Human LIN54 (a.a. 555-631) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Recombinant human LIN54 protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 555-631 aa of BC109278

Conjugate

Application Notes

WB: 1:500-1:2000

General Notes

This product (Catalog No. DPABH-01393) is a rabbit-derived polyclonal antibody raised against human LIN54, targeting the amino acid region 555–631 of the protein. It has been affinity purified by Protein A. LIN54 is a component of the LINC/DREAM complex, an important regulator of cell cycle genes (PMID: 17671431; 19725879). The complex binds to more than 800 promoters in G0 phase and is required for repression of E2F target genes (PMID:17531812). In S phase, the complex binds to the promoters of G2/M genes whose products are required for mitosis and plays an important role in their cell cycle dependent activation (PMID:17671431). LIN54 has a DNA binding region (CXC-hinge-CXC, CHC domain), which consists of two cysteine-rich (CXC) domains separated by a short spacer. It has been reported that the CHC domain functions as a DNA binding domain, and conserved cysteine residues in two CXC domains are essential for DNA binding (PMID: 22895175). The calculated molecular weight of LIN54 is 79 kDa and an apparent molecular mass of 100-110 kDa has been reported (PMID: 17531812; 28920576; 17671431).
DPABH-01393 is suitable for use in Western blot (WB) (1:500–1:2,000) application.
DPABH-01393 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

CXCDC1; JC8.6; KIAA2037; lin 54 homolog (C. elegans); LIN54; MIP120; Protein lin 54 homolog

Entrez Gene ID

UniProt ID