Anti-LIMK1 monoclonal antibody (DCABH-928)

Mouse anti-Human LIMK1 monoclonal antibody for WB, ICC/IF Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2b
Clone
4H4
Species Reactivity
Human
Immunogen
Recombinant full length protein of Human LIM Kinase 1 produced in HEK293T cells (NP_002305).
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/2000; ICC/IF: 1/100;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
LIMK1; LIM domain kinase 1; LIMK; LIM motif-containing protein kinase; LIMK-1
Entrez Gene ID
UniProt ID

Product Background


Gene summary
LIMK1 (LIM Domain Kinase 1) is a Protein Coding gene. Diseases associated with LIMK1 include williams-beuren syndrome and supravalvar aortic stenosis. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Signaling by GPCR. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TESK1. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Antigen Description
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11. 23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. LIM domain kinase 1 is an enzyme that in humans is encoded by the LIMK1 gene. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs, a central PDZ domain, and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. The function about LIMK1 antigen include ATP binding; heat shock protein binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; zinc ion binding.
Pathway
Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; CDC42 signaling events, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem.

Citations


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References


Matsui, S; Matsumoto, S; et al. LIM kinase 1 modulates opsonized zymosan-triggered activation of macrophage-like U937 cells - Possible involvement of phosphorylation of cofilin and reorganization of actin cytoskeleton. JOURNAL OF BIOLOGICAL CHEMISTRY 277:544-549(2002).
Bagheri-Yarmand, R; Mazumdar, A; et al. LIM kinase 1 increases tumor metastasis of human breast cancer cells via regulation of the urokinase-type plasminogen activator system. INTERNATIONAL JOURNAL OF CANCER 118:2703-2710(2006).

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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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