Anti-KPNB1 monoclonal antibody, clone 4F0

Antibody Isotype

IgG2a

Clone

4F0

Species Reactivity

Mouse; Rat; Cow; Dog; Human; Pig; African green monkey; Syrian Hamster

Immunogen

Full length native protein (purified) corresponding to Cow NTF97/Importin beta. Purified from Bovine erythrocytes.

Conjugate

Application Notes

Flow Cyt: 1/100; WB: 1/5000; ICC/IF: 1/1000.

Alternative Names

KPNB1; karyopherin (importin) beta 1; IMB1; IPO1; IPOB; Impnb; NTF97; importin subunit beta-1; PTAC97; importin 1; importin 90; importin-90; nuclear factor p97; importin beta-1 subunit; karyopherin subunit beta-1; pore targeting complex 97 kDa subunit;

Entrez Gene ID

UniProt ID

Antigen Description

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.

Pathway

Activation of DNA fragmentation factor, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem.