Rabbit Anti-KDM8 Polyclonal Antibody (DPABH-03706)
Rabbit Anti-Human KDM8 Polyclonal Antibody for WB, IHC
Bring this labeled antibody directly to your bench!
Add to basket
Human, Mouse, Rat
Recombinant funsion protein containing a sequence corresponding to amino acids 147-416 of human KDM8 (NP_079049.2).
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of extracts of various cell lines, using KDM8 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human mammary cancer using KDM8 antibody at dilution of 1:100 (40x lens).
KDM8; lysine (K)-specific demethylase 8; JMJD5; lysine-specific demethylase 8; jumonji domain containing 5; jmjC domain-containing protein 5
Have you cited DPABH-03706 in a publication? Let us know
and earn a reward for your research.
Hsia, DA; Tepper, CG; et al. KDM8, a H3K36me2 histone demethylase that acts in the cyclin A1 coding region to regulate cancer cell proliferation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107:9671-9676(2010).
Klose, RJ; Yamane, K; et al. The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36. NATURE 442:312-316(2006).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.