Rabbit Anti-KDM8 Polyclonal Antibody (DPABH-03706)
Rabbit Anti-Human KDM8 Polyclonal Antibody for WB, IHC
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Human, Mouse, Rat
Recombinant funsion protein containing a sequence corresponding to amino acids 147-416 of human KDM8 (NP_079049.2).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of extracts of various cell lines, using KDM8 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human mammary cancer using KDM8 antibody at dilution of 1:100 (40x lens).
KDM8; lysine (K)-specific demethylase 8; JMJD5; lysine-specific demethylase 8; jumonji domain containing 5; jmjC domain-containing protein 5
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Wang, ZY; Wang, C; et al. Differential proteome profiling of pleural effusions from lung cancer and benign inflammatory disease patients. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1824:692-700(2012).
Youn, MY; Yokoyama, A; et al. JMJD5, a Jumonji C (JmjC) Domain-containing Protein, Negatively Regulates Osteoclastogenesis by Facilitating NFATc1 Protein Degradation. JOURNAL OF BIOLOGICAL CHEMISTRY 287:12994-13004(2012).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.