Rabbit Anti-KDM8 Polyclonal Antibody (DPABH-03706)

Rabbit Anti-Human KDM8 Polyclonal Antibody for WB, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant funsion protein containing a sequence corresponding to amino acids 147-416 of human KDM8 (NP_079049.2).
Conjugate
unconjugated

Applications


Application Notes
WB:    1:500 - 1:2000; IHC:    1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Western blot analysis of extracts of various cell lines, using KDM8 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human mammary cancer using KDM8 antibody at dilution of 1:100 (40x lens).

Target


Alternative Names
KDM8; lysine (K)-specific demethylase 8; JMJD5; lysine-specific demethylase 8; jumonji domain containing 5; jmjC domain-containing protein 5
Entrez Gene ID
UniProt ID

Citations


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References


Hsia, DA; Tepper, CG; et al. KDM8, a H3K36me2 histone demethylase that acts in the cyclin A1 coding region to regulate cancer cell proliferation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107:9671-9676(2010).
Klose, RJ; Yamane, K; et al. The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36. NATURE 442:312-316(2006).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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